Yo Oizumi

Professor Layton (voice)

Maki Horikita

Luke Triton (voice)

Saki Aibu

Emmy Altava (voice)

Nana Mizuki

Janice Quatlane (voice)

Atsuro Watabe

Jean Descole (voice)

Hochu Otsuka

Inspector Clamp Grosky (voice)

Iemasa Kayumi

Oswald Whistler (voice)

Fumiko Orikasa

Melina Whistler (voice)

Kikuko Inoue

Celia Raidley (voice)

Sumire Morohoshi

Nina (voice)

Shozo Iizuka

Curtis O'Donnell (voice)

Kenta Miyake

Marco Brock (voice)

Megumi Toyoguchi

Amelia Ruth (voice)

Jouji Nakata

Frederick Bargland (voice)

LiLiCo

Annie Dretche (voice)

Koichi Yamadera

Pierre Starbuck (voice)

Minoru Inaba

Don Paolo (voice)

Shirou Saitou

Inspector Chelmey (voice)

Mamiko Noto

Flora Reinhold (voice)

Rokuro Naya

Dr. Andrew Schrader (voice)