Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Jennifer Hale

Felicia Hardy / Black Cat (voice)

Rodney Saulsberry

Joe 'Robbie' Robertson / Security Guard / Paramedic (voice)

Ed Asner

J Jonah Jameson (voice)

Efrem Zimbalist Jr.

Otto Octavius / Doctor Octopus / Butler (voice)

Rue McClanahan

Mrs. Hardy (voice)

Steven Polinsky

Security Chief / FBI Agent (voice)

David Hayter

Brace Huntington (voice)

John Phillip Law

John Hardesky / The Cat (voice)

Nita Talbot

Anastasia Hardy (voice)

Roscoe Lee Browne

Kingpin (voice)

David Warner

Landon (voice)

Jack Angel

Nick Fury (voice)

Rachel Davies

Agent X (voice)

Gary Imhoff

Harry Osborn (voice)

Maxwell Caulfield

Alistair Smythe (voice)

Cannon Young

Child Silvermane (voice)

Leigh-Allyn Baker

Alisha Silvermane (voice)

Eddie Albert

Adam Toomes / Old Vulture (voice)

Richard Moll

Scorpion (voice)

Alan Johnson

Arthur Avis / Young Vulture (voice)

Sandra Bernhard

Sarah Baker (voice)

Joseph Campanella

Doctor Curt Connors (voice)

Townsend Coleman

Middle-Aged Silvermane (voice)

Jeff Corey

Silverman (voice)